Edited by Oral micro, 18 January 2005 - 08:08 AM.
DGGE and Smiling bands!
2 replies to this topic
Posted 18 January 2005 - 08:07 AM
Hi! we've just got some new DGGE apparatus for our lab and after several attempts at running gels of a 40-70% gradient we always seem to get really bad smiling of the side wells. We run it over night at about 66volts. Any ideas how we can stop the smiling?
Posted 28 January 2005 - 02:11 PM
Do you have the sides of the plate closed off with electrical tape while running the gel?