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DGGE and Smiling bands!


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#1 Oral micro

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Posted 18 January 2005 - 08:07 AM

Hi! we've just got some new DGGE apparatus for our lab and after several attempts at running gels of a 40-70% gradient we always seem to get really bad smiling of the side wells. We run it over night at about 66volts. Any ideas how we can stop the smiling? :blink:

Edited by Oral micro, 18 January 2005 - 08:08 AM.


#2 Gouwetonny

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Posted 19 January 2005 - 05:37 AM

Concentration salt in buffer?

Keep on smiling :)

#3 SeahAG

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Posted 28 January 2005 - 02:11 PM

Do you have the sides of the plate closed off with electrical tape while running the gel?




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