I am doing some work about FISH.I used a synthetic oligocleotide probe labelde
with DIG .The probe length is about 24 bases.And i am going to detect the
target s with fluorescent antibody bought from Roche.The hybridization buffer i
used before is ,50% deionized formimide;2*SSC;10%dextran sulfate and 20ng
Is there something inappropriate with the components i used.
Thanks very much