No replies to this topic

[wendy]

 
     Fang Yang

       
     Junior Member
                                   

                    Hi
                      I am doing some work about FISH.I used a synthetic oligocleotide probe labelde
                      with DIG .The probe length is about 24 bases.And i am going to detect the
                      target s with fluorescent antibody bought from Roche.The hybridization buffer i
                      used before is ,50% deionized formimide;2*SSC;10%dextran sulfate and 20ng
                      probe/20ul buffer.pH7.0.
                      Is there something inappropriate with the components i used.
                      Thanks very much