How do people get on cloning oligos for shRNA expression into shRNA expression vectors? Any tips or tricks? I've just been cloning a couple and it was very difficult!"
David
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Cloning into shRNA vectors
Started by expresson_help, Jan 18 2005 05:54 AM
2 replies to this topic
#2
biotin
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Posted 18 February 2005 - 05:32 AM
Just synthesize the oligos(sense and antisense) with two overhand ends as restriction enzyme site and anneal the two oligos. Treat it as common insert to clone under polIII promoter.
Hopes this help
cheers
Hopes this help
cheers
#3
fred_33
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Posted 01 March 2005 - 06:49 AM
hi
for improving efficency of cloning, and especially if you have compatible ends, you should order phosphorylated oligos.
for improving efficency of cloning, and especially if you have compatible ends, you should order phosphorylated oligos.
