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SDS-PAGE gel cracks


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#1 sci60141

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Posted 23 May 2002 - 09:41 AM

I have been running small peptides on 10%-20% Tris-tricine gradient gel and after framing it in cellophane, the gel cracks into many fragments.  How can I prevent this?

#2 Kara Lee

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Posted 07 June 2002 - 10:14 AM

I had this same problem. What worked well for me was to soak the gel in a solution of 3% glycerol/30% methanol overnight before drying. It almost never cracked after that.




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