Edited by zhgljj1998, 13 January 2005 - 11:52 AM.
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ligation question
Started by zhgljj1998, Jan 13 2005 06:48 AM
3 replies to this topic
#1
Posted 13 January 2005 - 06:48 AM
can anybody tell me, is it useful to run the ligation mixin the gel?really confused since somebody suggested to do this step. Also, the Insert : Vector (mol)=? be better, 1:1; 1:3; 3:1????????or depend on your stuff?thanks.
#2
Posted 13 January 2005 - 09:42 AM
Do you mean to run a gel using the ligation reaction? It won't hurt if you do so and may give you some idea whether your ligation is successful or not.
#3
Posted 13 January 2005 - 11:36 AM
yes, can we exactly make sure the ligation work well or not from gel with control? thanks,man.Do you mean to run a gel using the ligation reaction? It won't hurt if you do so and may give you some idea whether your ligation is successful or not.
#4
Posted 26 January 2005 - 03:01 AM
I do not think you can make sure of your ligation from the gel .I did like this,though I can see three different bands(insert,digested vector,and after that was another band,which seems to be the ligation),but I got no right colony !
But it will not give any side effect by doing that ,and the ligation mixture will be useless after transformation,so ,you can have a try .
But it will not give any side effect by doing that ,and the ligation mixture will be useless after transformation,so ,you can have a try .
