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How to prepare enough plasmid for transfection?


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#1 Daniel5306

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Posted 12 January 2005 - 03:51 PM

I want to know what kind of method usually be used to prepare enough plasmid for transfection. I usually used the mini plamid preparation column of Qiagen from 5ml medium, but just can be used for several transfection. I want to know a method to prepare larger amount of plasmid at a time. Thank your attention!

Daniel

#2 chandima

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Posted 12 January 2005 - 09:21 PM

hi,
i used the mtd. given in Maniatis lab manual. got a good yeild and no contaminating chromosomal DNA , very pure. its a bit long protocol but sure.
chandima

#3 pcrman

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Posted 12 January 2005 - 10:26 PM

Just do maxpreps using a maxprep kit. You can grow bacteria in any volume of LB.

#4 Daniel5306

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Posted 13 January 2005 - 07:59 AM

Thank your valuable advice!

Daniel




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