Posted 12 January 2005 - 02:25 PM
Posted 06 March 2005 - 05:02 PM
normally, i would prepare a 1.5% soldium alginate solution. you can add the powder to ultrapure water and use a magnetic stirrer to stir it until all powder dissolved. i left it to stir overnight though. after which, just autoclave the solution. that will be fine.
Posted 07 March 2005 - 07:00 AM
Thanks for your suggestion. I stirred and heated the solution (2%) for ~3 hours to get it into solution. I didn't
sterilize it as I wasn't sure how it would react to autoclaving. I'll try it though. I'm using this as a cell matrix for implantation
into rats; what are you using it for? Thanks again.
Posted 07 March 2005 - 05:19 PM
Basically i only used sodium alginate solution for seeding of scaffolds. First i resuspend cells in 1.5% steriled alginate solution. This is followed by seeding the cell-alginate mixture onto the scaffolds (that have been soaked in steriled calcium chloride solution for ~ 1min). When the calcium chloride comes into contact with alginate, they coagulate to form a gel-like substance, which in turn act as a matrix to attach the cells to the scaffolds.
In my case i need the alginate solution to be steriled and till now the protocol works fine for me. Hope it works well for you too!