Does anyone have a protocol for solubilizing alginic acid sodium salt? I have made a 2% solution in ultra-pure water but had to heat it to 70C to get it into solution. I would like to use it to make a matrix for cultured cells so am also interested in how to sterilize it. Thanks.
Chris
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#2
Sg_ACC
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Posted 06 March 2005 - 05:02 PM
Although my reply might be abit late to be of any help to you, i would still like to share my protocol.
normally, i would prepare a 1.5% soldium alginate solution. you can add the powder to ultrapure water and use a magnetic stirrer to stir it until all powder dissolved. i left it to stir overnight though. after which, just autoclave the solution. that will be fine.
normally, i would prepare a 1.5% soldium alginate solution. you can add the powder to ultrapure water and use a magnetic stirrer to stir it until all powder dissolved. i left it to stir overnight though. after which, just autoclave the solution. that will be fine.
#3
cchambers
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Posted 07 March 2005 - 07:00 AM
Sg_ACC,
Thanks for your suggestion. I stirred and heated the solution (2%) for ~3 hours to get it into solution. I didn't
sterilize it as I wasn't sure how it would react to autoclaving. I'll try it though. I'm using this as a cell matrix for implantation
into rats; what are you using it for? Thanks again.
Chris
Thanks for your suggestion. I stirred and heated the solution (2%) for ~3 hours to get it into solution. I didn't
sterilize it as I wasn't sure how it would react to autoclaving. I'll try it though. I'm using this as a cell matrix for implantation
into rats; what are you using it for? Thanks again.
Chris
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Sg_ACC
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Posted 07 March 2005 - 05:19 PM
Hello Chris,
Basically i only used sodium alginate solution for seeding of scaffolds. First i resuspend cells in 1.5% steriled alginate solution. This is followed by seeding the cell-alginate mixture onto the scaffolds (that have been soaked in steriled calcium chloride solution for ~ 1min). When the calcium chloride comes into contact with alginate, they coagulate to form a gel-like substance, which in turn act as a matrix to attach the cells to the scaffolds.
In my case i need the alginate solution to be steriled and till now the protocol works fine for me. Hope it works well for you too!
GooD LucK!
Basically i only used sodium alginate solution for seeding of scaffolds. First i resuspend cells in 1.5% steriled alginate solution. This is followed by seeding the cell-alginate mixture onto the scaffolds (that have been soaked in steriled calcium chloride solution for ~ 1min). When the calcium chloride comes into contact with alginate, they coagulate to form a gel-like substance, which in turn act as a matrix to attach the cells to the scaffolds.
In my case i need the alginate solution to be steriled and till now the protocol works fine for me. Hope it works well for you too!
GooD LucK!
