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DNA extraction from plasma/serum


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#1 mora

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Posted 12 January 2005 - 10:15 AM

Can someone help me with extraction of DNA from plasma/serum?
I tryied with Quiagen DNA blood midikit: very low DNA concentration.
Another question, it is better serum or plasma?

#2 nabla

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Posted 18 January 2005 - 06:23 AM

Do not you need lymphocytes? Therefore you need whole blood to get gDNA.

#3 Paja

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Posted 01 February 2005 - 03:32 AM

Hi Mora
I know it's possible to extract DNA from both serum and plasma, but have no personal experience with this. You don't need lymphs to get DNA - there is a free DNA in plasma. The question is of what quality and quantity... I am afraid you woun't get somehow good yields even when using Qiagen kits...
Sorry.
Paja




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