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How to insert 10His-TEV cassette upstream of coding sequence


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#1 Yakov

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Posted 10 August 2002 - 11:08 AM

Colleagues, could you share you approaches/experience in inserting 10xHis--TEV cassette upstream of coding sequence for a protein to be expressed (in yeast)?

#2 Wotan

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Posted 13 August 2002 - 08:03 AM

Try out using the Ex-Site-Mutagenesis-Kit from Stratagene, if there is no targeting-sequence (for example for mitochondrial transit)




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