We are trying to do Westerns and I have treated the sample with SDS/beta-mercaptoethanol. The protein size on the gel is approximately 46kDa but we can never see the 17kDa fraction.
Potentially I have 2 problems: 1) an antibody that does not work such that it is not specific; 2) an antibody that is correct but I cannot get the protein to fall apart.
Would you have any suggestions for alternatives on denaturing proteins?
Edited by scientist, 11 January 2005 - 10:47 AM.