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About 5' aza-cytidine


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#1 rocio

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Posted 11 January 2005 - 05:36 AM

Hi,
I'm starting to work whit 5' cytidine on plants. I want to know if it's possible to prepare a stock solution to store it at -20C. In that conditions, the drug lose the desmetilant capacity?
I've wrote to SIGMA but I haven't had any answer.

I'll appreciate any advise
rocio

#2 rajcell

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Posted 11 January 2005 - 10:27 AM

Dissove 5'aza-deoxycytidine in PBS at 10 mM, aliquot in small volume (100 ul) and store at -70C. After an aliquot has been thawed 3-4 times, don't use it anymore, discard it.

#3 rocio

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Posted 12 January 2005 - 01:31 AM

Dear rajcell:
Thanks to answer my question

rocio




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