Edited by bioforum, 14 January 2005 - 09:38 AM.
Why is DTT used in reverse transcription (RT) reaction?
Posted 11 January 2005 - 03:29 AM
Posted 12 January 2005 - 12:55 PM
Posted 14 January 2005 - 06:14 AM
Thank you for your reply. But normally there is no disulfide bond in the mRNAs, so i wonder.........
Dithiothreitol is a reducing agent, so it helps to break bonds (like disulfide bonds) which will loosen the secondard structure of the RNA and facilitate RT enzyme initiation of transcription and processivity.
Posted 14 January 2005 - 09:15 AM
Posted 15 May 2012 - 03:21 PM