Western Primary AB
Posted 11 January 2005 - 02:25 AM
Posted 11 January 2005 - 07:23 AM
I really can't recommend one way or the other. I would think that this is a matter of try and error. I use the 1h RT method myself most of the time with most antibodies, and get good results from that. A collegue of mine incubates his westerns at 4°C ON a gets also good results. So, both ways may work, but when in doubt, try it! When using commercially available Abs, have a look at the manufacturers datasheet and try what's recommended there first.
Posted 11 January 2005 - 11:52 PM