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Western Primary AB


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#1 Trinity

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Posted 11 January 2005 - 02:25 AM

Hi! I do some westernbotts. Is it more recommendable to incubate the primary antibody at 4 C overnight or 60 min at room temperature?

Tanks

#2 jadefalcon

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Posted 11 January 2005 - 07:23 AM

Hi!
I really can't recommend one way or the other. I would think that this is a matter of try and error. I use the 1h RT method myself most of the time with most antibodies, and get good results from that. A collegue of mine incubates his westerns at 4C ON a gets also good results. So, both ways may work, but when in doubt, try it! When using commercially available Abs, have a look at the manufacturers datasheet and try what's recommended there first.


mike
--- He who finds typos may keep them! ---

#3 serglom

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Posted 11 January 2005 - 11:52 PM

I incubate overnight or 2 hours at room temperature with good results. I have never incubated for 1 hour, but it seems to work too




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