A gene that I am working with shows promoter methylation (detected by both MSP and bisulfite sequencing) but I found that the mRNA of the gene is still expressed.
Does anyone know if there are exceptions for promoter methylation-mediated gene silencing?
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paulina
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Posted 08 January 2005 - 09:03 PM
How big is the area you have mapped using bisufite squencing? Have you covered the whole 5' CpG island of your gene? Usually, if a gene is expressed, there should be no methylation within the island, while methylation may exist outside of it.
