I am attempting to isolate DNA from picocyanobacteria for PCR. It can be successfully done with mechanical methods to open the cell wall e.g. glass beads. I however want to increase the sensitivity of the process so I can remove DNA from say 50 cells per ml.
Any Ideas? Freeze thaw, liquid N2 and boiling dont seem to work.
The problem is removing the cell wall.
Category: General method
Date: 14 Jun 1999
Remote Name: 220.127.116.11
I need a protocol to prepare G+A marker along with my footprinting experiment. I knowthere are some simplified protocols on this besides the original Maxim-Gilbert method.
Thanks in advance