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[Rachel]

Hi

I am attempting to isolate DNA from picocyanobacteria for PCR. It can be successfully done with mechanical methods to open the cell wall e.g. glass beads. I however want to increase the sensitivity of the process so I can remove DNA from say 50 cells per ml.

Any Ideas? Freeze thaw, liquid N2 and boiling dont seem to work.

The problem is removing the cell wall.

With Thanks

Katie

From: Luca
Category: General method
Date: 14 Jun 1999
Time: 11:55:34
Remote Name: 128.218.8.3

Comments

I need a protocol to prepare G+A marker along with my footprinting experiment. I knowthere are some simplified protocols on this besides the original Maxim-Gilbert method.

Thanks in advance

Luca