Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Hind III marker


  • Please log in to reply
1 reply to this topic

#1 virologist

virologist

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 07 January 2005 - 01:20 AM

Dear Friends
when ever i run hind iii marker with my pcr products , I get .5 Kb fragment well seperated but not bigger ones after running gel too long.
Please suggest what to do. Is there any method of making a marker.
Warm wishes

#2 Microman

Microman

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 07 January 2005 - 11:45 AM

Hi,

I have just started to do something similar, im not sure if this will answer your question but it may help.

I used the following


5ul lambda DNA (2ug/ul)
44ul React 2 Buffer
1ul Hind III

It took about 5hrs to digest the DNA. My digest gave me 7 bands of the following sizes (KB): 23.10, 9.40, 6.60, 4.40, 2.30, 2.00 & 0.56.

The 0.56KB band was very faint. I have used the above to quantify my PCR products.

Hope it helps




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.