i would like to clone my PCR product into the pCR-TOPO II vector from invitrogen. using the method recommended by the manufacturer ( only AMP in the LB/agar plate), i did get a few white clones. but when i extracted the plasmid from at least 5 clones and did restriction digestion, i could not find any insert there. it's said that the effienciency of TOPO CLONING is very high. the pCR-TOPO II vector is AMP and KANA double resistant. should i add KANA into the LB/agar plate when i steak the transformant? could anyone give me some advice on TOPO-cloning? appreciate your suggestion!
Edited by bioforum, 06 January 2005 - 05:37 PM.