Need to improve RNA integrity
Posted 06 January 2005 - 05:51 AM
Posted 06 January 2005 - 10:03 AM
The alternative is to pruchase RNeasy columns from Qiagen. I use them when preparing RNA for microarray and the yield puritiy ratios >1.9. You could always take your existing extractions and pass them though the columns, I believe there is a protocol in the QIagen manual for doing this.
The Biology Ninja
Posted 06 January 2005 - 03:07 PM
Posted 08 January 2005 - 01:07 PM
I called Qiagen tech support to see if I could omit the BME. They tell me it is neccessary for denaturing RNases. Unless I'm mistaken, BME reduces di-sulfide bonds largely. I don't know about it's ability to denature protein. They tell me I cannot substitute DTT for the BME...I don't know if I believe them but have yet to be adventureous enough to attempt using DTT in lieu. Anyone tried something besides BME?
DTT reminds me of popcorn. It's yummier to smell.
- The Biology Ninja