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Oligo ligation to PCR product

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#1 anonymous



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Posted 22 March 2001 - 10:00 PM

I am using a 2 % Agarose / 1 M urea gel for separation and purification of two RNA fragments 600 and 900 ribonucleotideslong.

I am using a new type of agarose called agarosee resolutefrom American Bioanalyticals. I get very inconsistent results sometimes the separation is very beautiful sometimes it lookssmeary and the yield is very low.

I was wondering if anyone has used this method, andif they have a good consistent approach to preparing andrunning the gels.


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