Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Oligo ligation to PCR product


  • Please log in to reply
No replies to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 22 March 2001 - 10:00 PM

I am using a 2 % Agarose / 1 M urea gel for separation and purification of two RNA fragments 600 and 900 ribonucleotideslong.

I am using a new type of agarose called agarosee resolutefrom American Bioanalyticals. I get very inconsistent results sometimes the separation is very beautiful sometimes it lookssmeary and the yield is very low.

I was wondering if anyone has used this method, andif they have a good consistent approach to preparing andrunning the gels.

Thanks!






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.