2 replies to this topic

[petraparker]

Hi!!
Can I do an Klenow-Polymerase and following SAP-treatment in the same buffer as my restiction digest?!
Restriction digest buffer conditions: 20mM Tris (pH 8.5); 20mM MgCl; 1mM DTT; 100mM KCl.
I heard that Klenow-Pol works pretty much in any buffer, is that right?!
Happy science!!

[newdragon]

Different enzyme needs different kind of circumstances.
you can find some information from NEB company.

newdragon

[tfitzwater]

Klenow and SAP will work in most restriction buffers.  When I cut vectors, I routinely perform the digestion in the presence of SAP.