Do enzymes work in different buffers?
Posted 05 January 2005 - 02:35 AM
Can I do an Klenow-Polymerase and following SAP-treatment in the same buffer as my restiction digest?!
Restriction digest buffer conditions: 20mM Tris (pH 8.5); 20mM MgCl; 1mM DTT; 100mM KCl.
I heard that Klenow-Pol works pretty much in any buffer, is that right?!
Posted 11 January 2005 - 02:55 AM
you can find some information from NEB company.
Posted 19 January 2005 - 04:28 PM