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#1 anonymous

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Posted 20 April 2001 - 09:00 PM

I always had good results using electroporation for P815. 350V 80uF 5 million cells in 0.5ml HBS let them rest for 15 min. after zapping, then put in T75 flask with about 25ml 10%FBS RPMI.

#2 anonymous

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Posted 20 April 2001 - 09:00 PM

Any tips/protocols on transfection (transient) of P815 mouse mastcytoma cell line and PHA blasts of monkey and humna PBMCs are needed. Protocols and ref will be highly appreciated.

Thanks,

Devesh


#3 anonymous

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Posted 24 May 2001 - 09:00 PM

Really depends on the cell type and culture conditions - GAPDH may be appropriate




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