Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

I want to learn "MSP"


  • Please log in to reply
2 replies to this topic

#1 pengzhl

pengzhl

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 02 January 2005 - 05:09 AM

I have done "MSP" for about 3 months,but untill now I do not get any rusults.I use CHMICON's DNA modification Kit,and use Hotstart PCR.BUT my PCR has not nothing except primer dimer,my primer has worked by others.
So I want to learn this method.If you will perform "MSP",please tell me!My phone number is 13187167226 .My QQ is 6117657. Please touch with me!A LOT OF THANKS!

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 02 January 2005 - 02:13 PM

Can you post your PCR conditions and your primer sequence?

Since you are using the chemicon kit, the problem may not be in the modification part. If you can get some working modified DNA from others, you can use that as positive control.

#3 pengzhl

pengzhl

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 02 January 2005 - 07:10 PM

Can you post your PCR conditions and your primer sequence?

Since you are using the chemicon kit, the problem may not be in the modification part. If you can get some working modified DNA from others, you can use that as positive control.

I performed Touchdown PCR:95-5m(1 cycle);95-40s,60-40s,72-40s(3 cycles);then annealing T is 58-40s(2 cycles),56-40s(2 cycles),54-40s(2 cycles),52-40s(2 cycles),50-40s(2 cycles),48-40s(30 cycles);finally 72-5m.
I now perform p16 and RASSF1A genes and the primers are all from the articles.
And in our lab,only me is performed "MSP".So it is more difficult to do this work.I really want to learn from you! THANKS!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.