Hiii All
Iam a project assistant working with plant genomic DNA and I encounter with the recurrent roblem with dna elution from agarose gel by the techniques like electroelution, Sucrose density gradient ultracentifugation.The concern is that I get a good quantity of dna after restriction digestion but when I run the samples after Sucrose density gradient ultracentifugation I dont find any Dna in the gel ,I want to know the ways to overcome this problem .the fragment size which Iam looking for is more than 5 KB. Is there anyother way to purify largefragement .
Please assist me in this
Warm regards
Jayakumar
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