What kind of method should be utilized to purify linearized plasmid for mammalian cell transfection? I usually used agrose gel and gel freeze squeeze column to purify the product, but I am not sure whether the contaminant from the gel (such as dye or EB) will affect the transfection effeciency or the cell or not! Are there some haveing such experience?
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purify linearized plasmid for transfection
1 reply to this topic
Posted 03 January 2005 - 08:13 AM
Any standard method for purifying linearized plasmid from the gel should be fine. I don't think EB or the dye should affect transfection, since they should be removed in the prep. I have used kits from Qiagen and they should work well for you. See the following URL: