PCR does not work
Posted 30 December 2004 - 01:50 PM
The forward primer: (AAAAAAAACATATG)CAGCGGGCGCGACCCACGCTCTGG:
Backward primer: (AAAAAAAAGGATCCCTA)CTTGCTCTGCATGCTGTAGC
The sequence in () is the part I want to insert to the PCR product and are not surpose to bind to the template. I tried anealing temperature 55 and 64 degree and I also tried different amount DNA template (1ng to 200ng). But I got no band.
could anybody tell me what may be the problem?
Posted 02 January 2005 - 04:30 AM
God helps them who help themselves!
Posted 02 January 2005 - 02:32 PM
Double check your primer sequence to be sure there is no errors. Also check if your template DNA is good.