Genomic DNA purification
Posted 29 December 2004 - 07:32 AM
This time, I have a set of Genomic DNA samples purified by QIAmp DNA Blood Mini Kit according to manufacturer's instructions. In some samples the 260/280 ratios are very low (some 1.26- some 0.88), in all the concentration os <0.01 ug/ul...
The company's troubleshooting is not very useful due to the fact that Protease is stored in aliquots, 100% alcohol was used, > 1 million cells were used for extraction, etc, etc, and more importantly, the sources of these samples are no longer available!! so this is ALL or NONE...
I was thinking in re-extract DNA by using this kit or speed-vac concentration...
What do you think/suggest?
I will REALLY appreciate you responses!!
Posted 29 December 2004 - 11:34 PM
What is the purpose of the DNA you isolated?
Posted 30 December 2004 - 12:19 AM
The second time I did no OD measuring, and the real time pcr made no problems though. So it depends on the application.
Posted 30 December 2004 - 04:19 AM
DNA will be used for PCR amlification.