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Genomic DNA purification


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#1 thegradstudent

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Posted 29 December 2004 - 07:32 AM

Warm Christmas Wishes and Happy 2005 to ALL!!

This time, I have a set of Genomic DNA samples purified by QIAmp DNA Blood Mini Kit according to manufacturer's instructions. In some samples the 260/280 ratios are very low (some 1.26- some 0.88), in all the concentration os <0.01 ug/ul...

The company's troubleshooting is not very useful due to the fact that Protease is stored in aliquots, 100% alcohol was used, > 1 million cells were used for extraction, etc, etc, and more importantly, the sources of these samples are no longer available!! so this is ALL or NONE...

I was thinking in re-extract DNA by using this kit or speed-vac concentration...

What do you think/suggest? :D

I will REALLY appreciate you responses!!

Thanks
TheGradStudent

#2 Gouwetonny

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Posted 29 December 2004 - 11:34 PM

Spectro analysis for genomic DNA is not always thrust worthy.

What is the purpose of the DNA you isolated?

#3 nabla

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Posted 30 December 2004 - 12:19 AM

I had a similar problem. So precipitated the samples by using standard protokoll (NaAc and EtOH). But what are you using th samples for?
The second time I did no OD measuring, and the real time pcr made no problems though. So it depends on the application.
Good luck!

#4 thegradstudent

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Posted 30 December 2004 - 04:19 AM

thanks for your replies...

DNA will be used for PCR amlification.
TheGradStudent




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