3 replies to this topic

[thegradstudent]

Warm Christmas Wishes and Happy 2005 to ALL!!

This time, I have a set of Genomic DNA samples purified by QIAmp DNA Blood Mini Kit according to manufacturer's instructions. In some samples the 260/280 ratios are very low (some 1.26- some 0.88), in all the concentration os <0.01 ug/ul...

The company's troubleshooting is not very useful due to the fact that Protease is stored in aliquots, 100% alcohol was used, > 1 million cells were used for extraction, etc, etc, and more importantly, the sources of these samples are no longer available!! so this is ALL or NONE...

I was thinking in re-extract DNA by using this kit or speed-vac concentration...

What do you think/suggest?

I will REALLY appreciate you responses!!

Thanks

[Gouwetonny]

Spectro analysis for genomic DNA is not always thrust worthy.

What is the purpose of the DNA you isolated?

[nabla]

I had a similar problem. So precipitated the samples by using standard protokoll (NaAc and EtOH). But what are you using th samples for?
The second time I did no OD measuring, and the real time pcr made no problems though. So it depends on the application.
Good luck!

[thegradstudent]

thanks for your replies...

DNA will be used for PCR amlification.