I have a vector pFLAG-CMV2, which is inserted a fragment cut with Cla 1 and Not 1. So, in The vector Multiple Cloning Site, the fragment between Cla 1 and Not 1 is missing.
I want to get a empty pFLAG-CMV2 as control, could anyone tell me the best way to clone a empty vector?
thanks in advance!
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Best way to get the vector
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