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DGGE and reamplification


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#1 drjjj

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Posted 29 December 2004 - 01:31 AM

Hi,

I am trying to reamplify PCR products extracted from DGGE gel slices but with no success. The extraction method I am using is incubating the gel slices containing the DNA in 50 microl. DDW overnight at 4C. I would very much appreciate any help you offer regarding this problem.

Thanks very much

#2 taggart

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Posted 23 February 2005 - 03:16 AM

Hi

We use the same overnight incubation in ddw but the next day we vortex the tube and prior to PCR stir the slice around in the water with a tip this works for us alternatively you could try freeze thaw treatment

good luck




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