2 replies to this topic

[Phoneman]

Hi everyone:

I want to do FACS analysis on tumors growing on mice now, I would like to check the distribution of diploid cells in tumor. It would be very appreciated if any one can give me some suggestions, espicially how could I make sigle cell suspension for later FACS analysis.

Thanks in advance!

[mehdiimani]

Phoneman, on Dec 27 2004, 07:31 AM, said:

Hi everyone:

I want to do FACS analysis on tumors growing on mice now, I would like to check the distribution of diploid cells in tumor. It would be very appreciated if any one can give me some suggestions, espicially how could I make sigle cell suspension for later FACS analysis.

Thanks in advance!

hi you must have suspension cells or homogenize your tissue

[wirly]

I think people usually use collagenase to free individual cells from tissue.  It is a bit tricky but with some practice it seems to work.