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FACS analysis for tissue samples?


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#1 Phoneman

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Posted 27 December 2004 - 06:31 AM

Hi everyone:

I want to do FACS analysis on tumors growing on mice now, I would like to check the distribution of diploid cells in tumor. It would be very appreciated if any one can give me some suggestions, espicially how could I make sigle cell suspension for later FACS analysis.

Thanks in advance!

#2 mehdiimani

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Posted 28 December 2004 - 10:17 PM

Hi everyone:

I want to do FACS analysis on tumors growing on mice now, I would like to check the distribution of diploid cells in tumor. It would be very appreciated if any one can give me some suggestions, espicially how could I make sigle cell suspension for later FACS analysis.

Thanks in advance!

hi you must have suspension cells or homogenize your tissue

#3 wirly

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Posted 10 January 2005 - 05:18 PM

I think people usually use collagenase to free individual cells from tissue. It is a bit tricky but with some practice it seems to work.




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