Posted 25 December 2004 - 06:59 PM
I used the calcium chloride protocol. Temperature of incubation? heat shock? amounts of cells/plasmid used?
Thanks in Advance,
Posted 26 December 2004 - 10:17 AM
Trying to incorporate various environmental resistance genes
Posted 27 December 2004 - 05:27 AM
There are different methods of E. coli transformation, including heat shock (which could be performed at 37 or 42 C degree) and electroporation.
It also strain and insert dependent, and if you use old cells it might also not work.
Posted 31 December 2004 - 09:09 PM
will CaCl2 mtd perform better to electrophoration in E.coli transformation. I tried electrophoration and always got blue colonies only
Posted 02 January 2005 - 11:22 PM
God helps them who help themselves!