Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Cell lysis when isolating dendritic cells

  • Please log in to reply
1 reply to this topic




  • Members
  • Pip
  • 1 posts

Posted 21 December 2004 - 02:33 PM

Hello everyone, I have a simple question regarding DC harvesting.

I currently use the plastic adherence technique when isolating DC precursors. So after the 2hr incubation, I am ready to pool the cells into one flask. However, it seems that if I use a cell scraper to harvest the cells, I observe cell lysis which results in big clumps of cells. Since I am working with a small amount of cells, I need everyone I can get. What is the best way to harvest the adherent cells from the flask that will result in minimal cell lysis?
These cells are isolated from peripheral blood and incubated in AIM V serum free media. Has anyone else had lysis problems when isolating DC precursors from peripheral blood?

Thanks for the help.


#2 thegradstudent



  • Active Members
  • PipPipPipPipPip
  • 40 posts

Posted 22 December 2004 - 03:49 PM

Instead of a scraper, do you think that incubation with trypsin for 5 min could solve your problem?

... no more than 5 min!!...

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.