i designed a primer for a promotor region of fcerg1 gene to study its snps effects via reporter gene study.
i did a epcr of the primers from ncbi and it matched perfectly to the corresponding locus, but after i added a restriction sites to the forward and reverse primer mul I and Xho I respecitve÷y for PCR cloning in my PGL2 basic vector, the primers dont score a epcr hit.
is this a serious problem.... will it affect the actual amplication of my product during real PCR.
please explain me how to proceed?
PCR Primer Design with restriction sites
1 reply to this topic