PCR - No bands anymore in well functioning PCR
Posted 21 December 2004 - 06:10 AM
Thanks in advance
Posted 21 December 2004 - 01:24 PM
or play with the Mg concentration... or alter the temps just a little (gradient machine)... try using a fresh batch of buffers and enzyme... let someone else make it up... put in more DNA.... put in less DNA...
Edited by vetticus3, 21 December 2004 - 01:30 PM.
Posted 22 December 2004 - 11:04 AM
Maybe playing with DNA amount can help.
Thanks a lot for the recommendations.
Posted 27 December 2004 - 11:21 AM
Mercifully free of the ravages of intelligence - Time Bandits
Posted 02 January 2005 - 10:53 PM
do you check whether heating of buffer in electrophoresis, if so change the buffers regularly and try one more time.
Is your EtBr is working properly, are exposed much time to light
Posted 02 January 2005 - 11:16 PM
God helps them who help themselves!