Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

breast cancer cell primary culture


  • Please log in to reply
2 replies to this topic

#1 steven

steven

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 19 December 2004 - 06:42 PM

Hi
Is there anyone has experience on the primary culture of breast cancer? could you provide the protocol how to set up this kind of primary culture? Thank you very much
steven

#2 DevGrp

DevGrp

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 20 December 2004 - 07:38 AM

I used to do breast primary culture. I'll see if I can find the protocol tonight amoung my old backups or in Electronic copies of my students thesis. Otherwise our group published it in:

Speirs V., Green A. R., Walton D. S., Kerin M. J., Carleton P. J., Fox J. N., Desai S. B. and Atkin S. L., Short-term primary culture of epithelial cells derived from human breast tumours. Br. J. Cancer,78(11):1421-9 1998

V. Speirs, A. R. Green and M. C. White, Collagenase III: a superior enzyme for complete disaggregation and improved viability of normal and malignant breast tissue. In Vitro Cell. Dev. Biol. 32 (1996), pp. 7274.

J. T. Emerman and D. A. Wilkinson, Routine culturing of normal, dysplastic and malignant human mammary epithelial cells from small tissue samples. In Vitro Cell. Dev. Biol. 26 (1990), pp. 11861194.

I've just checked and none of these seem to be online. can you get them from a library?

#3 DevGrp

DevGrp

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 22 December 2004 - 01:10 AM

Either I could email you our method or a similar method can be found in Pubmed paper

good luck


Ian




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.