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Autoclave alternative


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5 replies to this topic

#1 JMan

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Posted 17 December 2004 - 02:34 PM

Hello all. I was wondering if there is a good alternative to autoclaves for sterilizing flasks and such that are meant for growing bacteria. I am thinking some sort of chemical wash or something?
Just wondering

-JMan

#2 green

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Posted 17 December 2004 - 05:32 PM

How about UV lighting or 70% alcohol spray? Also there are plastic big flasks which are autoclavable.

Edited by green, 17 December 2004 - 05:33 PM.


#3 phage434

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Posted 19 December 2004 - 02:47 PM

Dry heat at 180C for several hours is an effective alternative. Watch out for caps, however. The orange Corning and similar 45mm caps for media bottles, specifically, melt.

#4 sharath

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Posted 25 February 2005 - 04:26 AM

Gamma sterilization is the ideal method of choice for best result.
Sharath B.

#5 bob1

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Posted 27 February 2005 - 12:38 PM

Hi

You could try a 1% solution of sodium metabisulphite, this is sometimes used to sterilise flasks for brewing and wine making, and seems to be pretty effective.

#6 b3ka

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Posted 01 March 2005 - 07:07 PM

You could try a 1% solution of sodium metabisulphite, this is sometimes used to sterilise flasks for brewing and wine making, and seems to be pretty effective.


1% Sodium met. is a good sterilizer but needs a lot of rinsing. Many home-brewers use either Iodophor or StarSan. As StarSan (a food-grade acid-based sanitizer) tends to leave a residue, I go for iodophor. It's claimed effective at 12.5ppm titrable iodine with a 60second contact time. I use about a couple of capfulls (10ml) in 25litre water.

Have a link about the pros and cons of various sterilization procedures.

http://www.hoslink.c...erilisation.htm


--
B3ka.
Lab grunt, geek and brewer.




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