Hello all. I was wondering if there is a good alternative to autoclaves for sterilizing flasks and such that are meant for growing bacteria. I am thinking some sort of chemical wash or something?
Just wondering
-JMan
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Autoclave alternative
Started by JMan, Dec 17 2004 02:34 PM
5 replies to this topic
#1
Posted 17 December 2004 - 02:34 PM
#2
Posted 17 December 2004 - 05:32 PM
How about UV lighting or 70% alcohol spray? Also there are plastic big flasks which are autoclavable.
Edited by green, 17 December 2004 - 05:33 PM.
#3
Posted 19 December 2004 - 02:47 PM
Dry heat at 180C for several hours is an effective alternative. Watch out for caps, however. The orange Corning and similar 45mm caps for media bottles, specifically, melt.
#4
Posted 25 February 2005 - 04:26 AM
Gamma sterilization is the ideal method of choice for best result.
Sharath B.
#5
Posted 27 February 2005 - 12:38 PM
Hi
You could try a 1% solution of sodium metabisulphite, this is sometimes used to sterilise flasks for brewing and wine making, and seems to be pretty effective.
You could try a 1% solution of sodium metabisulphite, this is sometimes used to sterilise flasks for brewing and wine making, and seems to be pretty effective.
#6
Posted 01 March 2005 - 07:07 PM
You could try a 1% solution of sodium metabisulphite, this is sometimes used to sterilise flasks for brewing and wine making, and seems to be pretty effective.
1% Sodium met. is a good sterilizer but needs a lot of rinsing. Many home-brewers use either Iodophor or StarSan. As StarSan (a food-grade acid-based sanitizer) tends to leave a residue, I go for iodophor. It's claimed effective at 12.5ppm titrable iodine with a 60second contact time. I use about a couple of capfulls (10ml) in 25litre water.
Have a link about the pros and cons of various sterilization procedures.
http://www.hoslink.c...erilisation.htm
--
B3ka.
Lab grunt, geek and brewer.