2 replies to this topic

[drqin]

My target gene is 2.3kb, seems too long to common PCR. with what methods or reagent i can PCR this gene out completely?
Thank you for you reply.

[littlecell]

what's your template? genomic DNA or cDNA? I expand recommend high fidelity PCR system from ROCHE.

[katika]

You can use a long and accurate PCR that easy amplify up to 7 kb.  For example BD Clontech provides a kits (Advantage 2 PCR Kit) to perform such PCRs.