I am isolating RNA from bacteria grown in the 200ml of culture, I know the degradation of RNA will be avoided as possible if i can haveste the cell in a very short period , but the problem here is that the cell was grown in a large volume culture (for guarantying high yield of RNA),so it takes long time to centrifugate the cell (at least 15minte by a big motor).
I hope to get same suggestion on how to satble the RNA of bacteria.
Thanks in advance.
weimin
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kean_guy
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Posted 17 December 2004 - 12:37 AM
There shouldn't be a problem during 15' centrifugation. The cell won't lyse easily if you spin it at low speed (5000 to 7500 rpm) and at room temp. (or 4 C).
You should only start to worry after you've lysed your cell as RNase will be available in the solution along with your RNA.
You should only start to worry after you've lysed your cell as RNase will be available in the solution along with your RNA.
Edited by kean_guy, 17 December 2004 - 12:37 AM.
