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Autoclave to kill RNases/DNases?


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#1 LabGirl

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Posted 14 December 2004 - 03:26 PM

Due to the numerous contrary opinions amongst people in my lab, I have decided to put this question on the forum: does autoclaving destroy DNases and RNases? Half the people I know say it does, half say it doesn't....does anyone know definitively? Thanks :)

#2 paulina

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Posted 14 December 2004 - 03:49 PM

If it dose kill RNase, why we need to treat water with DEPC and then autoclave it?

Autoclaving is not effective at eliminating RNase in solution because the RNases simply renature as the solution cools.

Read this http://www.ambion.co.../tb/tb_178.html

#3 LabGirl

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Posted 15 December 2004 - 12:49 PM

I agree with the RNase issue (though other stubborn individuals do not), but I was a little less sure regarding DNases....




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