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Binding of protein to immunoassay plates


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#1 toto

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Posted 14 December 2004 - 02:09 AM

Hi,


Does anybody knows wether it is possible to coat protein on immunoassay plates(NUNC maxisorb) under denaturing conditions (typically few M urea or few M guanidine HCL)? I have a big doubt about that.

Thanks




:o

#2 jadefalcon

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Posted 16 December 2004 - 04:54 AM

usually you don't have harsh denaturating condition while coating, since you're dilluting the protein solution in coating buffer
I successfully coated protein denatured in 8M urea to medium-binding-capacity plates as well as to maxisorp plates. but I used only 2Ál of protein solution with 98Ál coating buffer, so the actual urea concentration was only 160mM while coating. worked fine in my hands.

mike
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