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Stability of DNA at 4 degrees?


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#1 Boo

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Posted 14 December 2004 - 01:03 AM

Just a simple question :o - what is the stability of extracted DNA at 4 degrees?

Is it better to keep at -20 when you are using it every day? I wanted to avoid numerous freeze/thaw cycles.

Or is it fine in the fridge for a week or two?

#2 Janina

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Posted 14 December 2004 - 02:05 AM

In general, DNA can be stored at 4 C, either in buffer or water... But this depends on your DNA. E.g., some viral DNA is not stable in the fridge.

#3 nabla

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Posted 14 December 2004 - 02:27 AM

I use genomic DNA over weeks, even several month (4 C: pcr positve control). Only pcr and sequencing products are stored at -20C when it takes more than one day to proceed with sequencing analysis.

#4 Boo

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Posted 14 December 2004 - 07:57 PM

Thanks :)




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