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tracking 150kDa protein through tissue

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#1 cellcount



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Posted 13 December 2004 - 08:48 AM


I want to assess the diffusion of proteins through muscle tissue. A 150kDa protein with a pI of about 6 should be monitored on its way through the tissue and its distribution should be assessed by cryosectioning. A pre-labelled protein would be useful or one that may be immunolabelled after fixation. I have no idea what I can use and hope some of you out there have a suggestion.

Thanks in advance

#2 bob1


    Thelymitra pulchella

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Posted 15 December 2004 - 04:32 PM


Check out Molecular Probes website (www.probes.com), they have a vast array of different probes for different purposes, which can be visualised by a range of techniques.

Hope this helps

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