Reamplification of PCR products
Posted 13 December 2004 - 05:41 AM
Could someone help me out with this. I want to know if reamplfication of PCR products will affect the mutational studies carried out later on these products. I need to reamplify because I have very little initial DNA templete.
Posted 14 December 2004 - 07:52 PM
Potentially there is an issue with repetitive amplification of PCR products, although this depends on the length of the product as to the likelihood of a mutation occuring in the PCR. If you are concerned about this you can get polymerases, such as Pfu, which have proofreading capability.
Posted 15 December 2004 - 11:35 PM
Thanks very much. Pfu would be better, why take chances.