chemical synthesized siRNA and rapidly dividing cells
Posted 11 December 2004 - 08:21 PM
I am new to RNAi area. Your suggestion and opinion are very important to me
If i am to use some rapidly dividing cells (e.g. HEK 293 cells) to perform RNAi study, is it not wise to use chemically synthesized siRNA?
I have this wonder because in this case the RNAi effects may be too insignificant to be detectable.
Is my concept correct? since i think for chemically synthesized siRNA, its RNAi signal cannot be replicate inside cells, and thus only cells transfected with siRNA will have effect. But new cells already grow if cells are rapidly dividing, leading to insignificant RNAi level.
Please let me know if my concept is correct. Thank you very much!
Posted 13 December 2004 - 10:13 AM
For those fast growing cells, I usually do the transfection immediately after I plate the cells or a couple of hours after the cells have settled.
Edited by paulina, 13 December 2004 - 10:13 AM.