We have different controls of methylation : controls unmethylated, controls 100 % methylated and controls partially methylated.
For controls unmethylated and 100% methylated, we obtain one PCR band specific and good sequencing results.
For controls partially methylated, we obtain 2 bands PCR (one specific and one unspecific) and very bad sequencing results.
What do you think ?
Edited by aurelie, 10 December 2004 - 05:44 AM.