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primary keratinocytes - HeKn - Cell Biology - BioForum

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primary keratinocytes - HeKn


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2 replies to this topic

#1 bionick

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Posted 10 December 2004 - 02:53 AM

Hello!
Somebody knows how cultre these cells? We are using original, recommended medium, but these cells growth very, very slowly (I've been waiting 3 months to get monolayer in T75 bottle), after trypsinisation 50% of cells are die. What I have to do to change this stuation?
I'll be gratefull for any tips, ideas, etc.
Thanks

#2 sea_liu

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Posted 10 December 2004 - 04:41 AM

perhaps this website may help you http://www.cascadebi...test&series=429

#3 joy_maf

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Posted 10 December 2004 - 05:30 PM

We add 10 ng/mL KGF (from Epoch Biolabs) in keratinocyte growth medium that help a lot. But be careful, KGF from other vendors sometimes have quality problem (variable from batch to batch).




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