2 replies to this topic

[shital]

Hi!!
             I was doing stable transfection. While picking up the clones from the 60 mm dishes to 96 well plate by single cell dilution I have  got contamination. Is there any way to remove them now? I have started penstrep (1x concentration)

[kant0008]

Hi,
sorry to tell you this, but contamination (bacterial, fungal, micoplasama) is VERY hard to remove, if not impossible! you can try harsh antibiotics, nut it might not work, or your cells will die..

[Jacen]

Plus, antibiotics may have an effect on your cell line that could make reproduceability a problem later on.  Best to start over.