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Cell contamination, needs the help urgently


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2 replies to this topic

#1 shital

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Posted 09 December 2004 - 04:03 PM

:(
Hi!!
I was doing stable transfection. While picking up the clones from the 60 mm dishes to 96 well plate by single cell dilution I have got contamination. Is there any way to remove them now? I have started penstrep (1x concentration)

#2 kant0008

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Posted 09 December 2004 - 09:39 PM

Hi,
sorry to tell you this, but contamination (bacterial, fungal, micoplasama) is VERY hard to remove, if not impossible! you can try harsh antibiotics, nut it might not work, or your cells will die..

#3 Jacen

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Posted 10 December 2004 - 05:50 AM

Plus, antibiotics may have an effect on your cell line that could make reproduceability a problem later on. Best to start over.




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