Bringing tumor line from animal back to cell culture
Posted 07 December 2004 - 07:35 AM
Thanks in advance.
Posted 07 December 2004 - 09:51 AM
Posted 07 December 2004 - 09:54 AM
Yes, I'm trying to isolate the cell line from a tumor fragment. What enzymes would you recommend? As of right now, the tumor fragment is laying down a base of many different cells, fibroblasts, etc. I am hoping that some of the tumor cells begin to adhere or bleb off and maintain in suspension.
Are you trying to get a cell line from the lymphoma tissue? If so, perhaps you could incubate the tissue with cell dissocation enzymes. If you must keep media on the tissue at all times, some enzymes do work, such as collagenase (but you may need more than just collagenase).
Edited by Jacen, 07 December 2004 - 02:34 PM.