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efficiently transfect macrophage


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#1 Daniel5306

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Posted 06 December 2004 - 09:38 AM

Does anybody know how to transfect macrophage? Now I am transfect J774A.1 and RAW264.7 with lipofectine from Invitrogen, but the transfection efficiency was very low. I want to know some method that can transfect macrophage efficiently and can get stable cell line. Any advice was appreciated.

Daniel From UNL

#2 erini

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Posted 29 December 2004 - 01:34 PM

you can use either retrovirus or adenovirus. Infection with retroviral vector will allow for stable expression of your gene, whereas adenoviral construct will ensure transient expression.

#3 wirly

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Posted 10 January 2005 - 05:51 PM

If you have access to an electroporator, electroporation works in almost every cell line but takes some monkeying to get the right conditions if you cannot find some already published. I have used is for cell lines that chemical tranfectants could not give stable transfections of.

I use a BTX unit and they have a lot of references to protocols for various cell lines. You can try some versions of their protocols.

http://www.btxonline...ian/Default.asp

good luck!




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