Help, macrophage transfection
Posted 06 December 2004 - 09:29 AM
Daniel From UNL
Posted 06 December 2004 - 09:38 PM
In general, most suspension cells are resistant to transfection by any other technique.
Posted 07 December 2004 - 08:13 AM
But the cells what I will use (J774A.1 and RAW264.7) were adhesive cell, which attach to the wall of the plate so firm that I have to use scraper when I subculture the cell. When I check the lipofectamine 2000 (invitrogen), the cell lines that can be transfected contained RAW264.7, but when I check the information of gencarrier 1/2, the information said they are better than lipofectamine. Some information indicated the Fugene6 might be better. It makes me fell difficult to decide which product I should try.
Thank you anyway!
Daniel from UNL
Edited by Daniel5306, 07 December 2004 - 08:16 AM.